<i>OK Clon</i> DNA 连接试剂盒 III Ver.2 - 图片 2

OK Clon DNA 连接试剂盒 III Ver.2

产品货号：AG11811 / AG11812

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规格

10 rxns / 10 μl

50 rxns / 10 μl

清除

价格范围：¥680 至 ¥2,600

产品描述

OK Clon DNA 连接试剂盒 Ⅲ Ver.2 是一种简单、快速、高效的定向无缝克隆试剂盒，可以快速高效地将一段或多段 DNA 片段定向、无缝地克隆到任意载体的任意位置，而不被酶切位点限制。本产品作为新一代无缝克隆试剂盒，适用范围广，可高效连接不同大小的载体与插入片段，尤其适用于多片段无缝克隆。
DNA 连接反应是基于 OK Clon Enzyme Ⅱ 的催化，将含有与载体两末端一致序列（15 ~ 20 bp）的片段准确、高效地插入到线性化载体中。只需在设计目的片段 PCR 引物时将与载体 5’ 端互补的序列添加在引物 5’ 端，通过该引物扩增目的片段，无需对 PCR 片段进行限制性酶切、磷酸化等处理，就可将目的片段插入载体中。

产品优势

① 本产品可将片段插入载体的任意位置，而不受酶切位点限制。
② 本产品反应效率高，单片段连接反应 10 分钟，多片段连接反应 20 分钟，即可完成连接反应。
③ 适用范围更广，可以对不同大小的载体及片段进行连接，特别适合于多片段的连接。
④ 插入片段不需酶切处理，PCR 扩增完成后只需要进行纯化处理便可用于连接反应。

产品组成